Soil water dye tracing,with special reference to the use of rhodamine WT,lissamine FF and amino G acid     

S. T. Trudgill 《水文研究》1987,1(2):149-170

Three fluorescent dyes (Rhodamine WT, Lissamine FF and Amino G Acid) are compared for use in soil water tracing. Severe limitations are evident, but practical applications are possible. Background fluorescence, adsorption, desorption, pH and other non-adsorptive effects are reviewed in the contexts of soil column work and field tracing of soil water. Lissamine FF and Amino G Acid are to be preferred for soil column work because of their lower adsorption; Rhodamine WT exhibits higher adsorption but is useful in field situations where organic fluorescence backgrounds are high. Semi-quantitative work may be undertaken in soil columns once a priming and flushing procedure has been adopted.  相似文献   

Biliary fluorescent aromatic compounds (FACs) measured by fixed wavelength fluorescence (FF) in several marine fish species from the NW Mediterranean     

David Insausti  Maite Carrasson  Francesc Maynou  Joan E. Cartes  Montserrat Sol 《Marine pollution bulletin》2009,58(11):1635-1642

The fixed wavelength fluoresce (FF) method was used to estimate the levels of fluorescent aromatic compounds (FACs) in the bile of fourteen fish species of commercial and/or ecological interest. Sampling was carried out in the NW Mediterranean at depths ranging from 50 to 1000 m during four seasonal cruises. During the summer sampling period, some species were also collected from another site (Vilanova fishing grounds) for comparison. Baseline levels of the polycyclic aromatic hydrocarbons (PAHs) naphthalene, phenanthrene, pyrene and benzo[a]pyrene were measured. Some seasonality was observed, with reduced FF levels in summer and no differences among sites, consistent with sediment PAH levels. We discuss our results in relation to fish phylogeny, season, depth, diet, trophic level and swimming capacity. Overall FF levels indicated differences among species; the suprabenthic feeders from shallow and deep communities, and Mullus barbatus in particular, displayed elevated FF values and are potential candidates for additional monitoring studies.  相似文献   

神经肽FF在小黄鱼(Larimichthys polyactis)性腺发育早期的表达与定位分析     

肖燃  宋长普  朱阳  黄伟  郑利兵  迟长凤  楼宝 《海洋与湖沼》2021,52(1):213-221

神经肽FF是属于RFamide相关肽家族中的一种八肽,在多种生物过程中具有调节功能。本文开展了小黄鱼(Larimichthys polyactis)神经肽FF相关基因(LpNPFF:Accession No.MT012894)的组织表达与定位分析研究。实时荧光定量PCR结果显示,小黄鱼神经肽FF基因在性未成熟期(第Ⅱ期)的小黄鱼的脑和性腺中表达较高,其次是肌肉和脾脏,在胃和肠中表达相对较少;原位杂交结果显示,在小黄鱼中脑的视顶盖(OTec)、圆环体(TL)、结节前核(NAT)、外侧结节核(NLT)、下丘脑弥散核(NDLI)以及延脑(MO)中检测到大量LpNPFF mRNA阳性的细胞,而端脑和小脑部分的表达较少。同时,在小黄鱼第Ⅱ期卵巢细胞的细胞质和核仁中也能观察到明显的阳性信号;免疫组织化学结果显示,在小黄鱼脑部表层细胞和卵巢壁周围的结缔组织中可见明显的RFamide免疫阳性纤维。上述研究结果有助于神经肽进化的深入研究,也为进一步探索NPFF相关基因在生殖调控方面的潜在功能提供了新的思路。  相似文献